In practice and despite Anfinsen’s dogma. the refolding of recombinant multiple SS-bonded proteins is famously difficult because misfolded species with non-native SS-bonds appear upon the oxidization of their cysteine residues. On the other hand. single SS-bond proteins are thought to be simple to refold because their cysteines have only one SS-bond partner. https://pipingrockers.shop/product-category/krill-oil/
Misfolding of a Single Disulfide Bonded Globular Protein into a Low-Solubility Species Conformationally and Biophysically Distinct from the Native One
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